James Watson and Francis Crick won the Nobel Prize in 1962 regarding the model of integrating DNA structures. Together with Rosalind Franklin and Maurice Wilkins, they stated that the structure of DNA is two nucleotide strands called the double helix, where the strands are arranged in a spiral position of sugar and the phosphate group is located on the outside and nitrogen bases are paired on the inner side ( connect the two strands). Apart from that, you might also want to check out the best lab equipment from biologia molecular.
Keep in mind that these bases do not pair randomly. Erwin Chargaff examined further the bases contained in DNA. He stated that the percentage of adenine (A) in DNA is always the same as the percentage of thymine (T), and the percentage of guanine (G) is always the same as the percentage of cytosine (C).
The description expressed by Watson and Crick explains the results of this study by estimating that base A always pairs with base T while base G always pairs with base C in the DNA chain. Therefore, A and T and G and C, are base pairs. If one DNA chain is encoded ATGCCAGT, then the pairing chain is TACGGTCA.
DNA Replication / Duplication
The sequence of base pairs is very decisive in the process of DNA replication. Duplication of DNA is the process by which DNA is imitated to form new DNA with the same genetic code.
During multiplication, the DNA chain is open (because weak hydrogen bonds are broken), so that inter-base bonds in the structure of the DNA molecule are broken. From each of these DNA strands (hereinafter referred to as mold threads) then the new nucleotides are synthesized based on the sequence of bases in the mold thread. The new nucleotide chain has a sequence of bases that are complementary to the base sequence in the mold thread.
Then, a pair of DNA strands (new DNA strands + new DNA strands) are bound together to form a new whole DNA thread and have the same genetic code as the previous DNA double thread. So, the multiplication process produces two of the same DNA molecules, namely one old and new double DNA strand. Therefore, this process is known as semiconservative replication because one DNA chain produces the same new DNA chain.